RG2550 Low E string tuning issue
I have been playing my 2550 a lot this past week, but I noticed something odd:
I had changed the strings awhile ago, and swiftly put it in the hardcase (I was busy playing my Carvin and Roadcore). I took out the 2550 to play and my tuning was a bit off. I did lots of stretching and now all the strings are fine, even when I pull back on the trem arm.. .except the low E string. It goes out of tune REALLY easily, and no matter how much I use the arm, the tuning isn't stable at all. I did lots of pulling on the arm to help stretch the strings, then I did 1.5-2 semitone bends to get more stretch going on, but it still goes way out of tune.
What are some possible issues that I can check and eliminate one by one until I find the cause of this issue?